Background: Taking account from the National Crime Records Bureau there were 31,677 cases of sexual assault registered in the single year 2021, which implies roughly 80 cases daily. To address such serious crimes forensic professionals must be aware and focus on traditional pieces of evidence such as DNA, Seminal Stains, bitemarks or struggle marks, and other confirming shreds of evidence. Bite mark analysis is a vital component of forensic investigations, offering a distinct form of evidence in criminal proceedings. By leveraging the uniqueness of dental structures, bite marks can contribute significantly to personal identification and the resolution of crimes involving assault, sexual offenses, and homicide. Main Body: We portrayed the collection, packaging, and preservation methods of bitemark, and included the DNA extraction from saliva. Structural distinctive features which are used to differentiate human dentition from animal dentition. Accidental and intentional crimes then easily distinguished. In the case of wildlife forensics also bitemarks have wide scope and importance for determination of species and tagging them with their habitat. Short Conclusion: In recent times with rapid evolution in computational capabilities, we are able to use complex applications of computer science for the purpose of three-dimensional comparison of bitemarks, use of digital image processing and image modifications. the statement on which most of us will agree is Bite marks are always being interest of investigators in cases of sexual assault and child abuse.